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. 2015 Oct 2;11(10):e1005196. doi: 10.1371/journal.ppat.1005196

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© 2015 Ye et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A-G) Suppressor activity of AS2, AS2-NES, AS2-NLS and AS2 I88A mutant. N. benthamiana (line 16c) leaves were co-infiltrated with 35S:GFP and 35S:AS2 or 35S:AS2-NES or 35S:AS2-NLS or 35S:AS2I88A (local silencing, A-D). E-G show systemic silencing. AS2 can interfere with systemic silencing induced by 35S:GFP (compare 5F of 35S:AS2 co-infiltrated with 5E of mock co-infiltrated with 35S:GFP and 5G with AS2 I88A mutant). Bar = 10 mm. Bar = 10 mm. (H) Western blot analysis showing GFP protein levels in N. benthamiana leaf (line 16c) infiltrated with 35S:GFP along with 35S:AS2, 35S:AS2-NES, 35S:AS2-NLS, 35S:AS2I88A, or mock treatment (upper panel). Coomassie blue-staining of the large subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcL) is shown as a loading control (lower panel). (I) Western blot analysis showing AS2 and its variants protein levels in N. benthamiana leaf. Coomassie blue-staining of the large subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcL) is shown as a loading control (lower panel).