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. 2015 Oct 2;10(10):e0139733. doi: 10.1371/journal.pone.0139733

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© 2015 Pooja et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (a) Quantification of benzyl penicillin (BP)‒treated and untreated S. agalactiae internalization into H9C2 cells. Q3‒uninfected population of H9C2 cells (blue dots); Q4‒population of H9C2 cells internalized with AO‒stained S. agalactiae (green dots) (b) Cytotoxicity analysis of BP-treated and untreated S. agalactiae on H9C2 cells. S. agalactiae grown in the presence of benzyl penicillin showed reduced cytotoxicity in H9C2 cells compared to untreated control (c) Determination of LTA-induced cytotoxicity in H9C2 cells after 6 h by MTT assay. Statistically significant differences are indicated by an asterisk (* p<0.05 or **p<0.01).