Table 1. List of primers used in the study.
| Gene name & Symbol | Primer Sequences (5’-3’) | |
|---|---|---|
| Semi-quantitative RT-PCR | RT-qPCR | |
| Tubulin, beta 5 class I (Tubb5) | F: CACAGGTGGCAAGTATGTCC R: GGTCTCATCCGTGTTCTAACC | F: CTGGGACTATGGACTCCGTT R: ACCAACTCAGCTCCCTCTGT |
| Actin, alpha 1, skeletal muscle (Acta1) | F: GACCACAGCTGAACGTGAGA R: GACCACAGCTGAACGTGAGA | F: CCAGAGTCAGAGCAGCAGAC R: CGTGTGGCTCAGTAGGAGAG |
| Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) | F: CGTCTTCACCACCATGGAGA R: CGGCCATCACGCCACAGTTT | F: CACCATCCGGGTTCCTATAA R: AGGGAGGAGCAGAGAGCAC |