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. 2015 Oct 2;11(10):e1005195. doi: 10.1371/journal.ppat.1005195

Fig 3. Treatment of NOKs-Akata cells with the differentiating agents, TPA and calcium chloride/serum, results in lytic EBV reactivation.

Fig 3

A) NOKs-Akata cells were treated with either TPA (20 ng/mL in K-SFM) or calcium chloride plus serum (1.2 mM CaCl in RPMI + 10% FBS) for 48 hours. Immunoblot analysis was performed to compare the levels of lytic viral proteins, Z and BMRF1, a differentiation-dependent cellular protein, involucrin, and the cellular protein, GAPDH (a loading control). B) NOKs-Akata cells were treated with TPA in the presence or absence of a ROCK inhibitor (Y27632) (10 μM) for 48 hours and immunoblot analysis was performed to compare the levels of Z, BMRF1 and involucrin. The cellular protein tubulin was used as a loading control.