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. 2015 Oct 2;11(10):e1005195. doi: 10.1371/journal.ppat.1005195

Fig 5. Endogenous KLF4 is required for TPA and sodium butyrate mediated lytic EBV reactivation in epithelial cell lines.

Fig 5

EBV-positive CNE-2 Akata cells were transfected with either control siRNA or two different KLF4 siRNAs, and then treated with TPA (20 ng/uL) (A) or sodium butyrate (3mM) (B) for 48 hours. Immunoblot analysis was performed to detect expression of endogenous KLF4, and lytic viral proteins Z, R and BMRF1. Actin served as a loading control. C) NOKs-Akata cells, transfected with either control siRNA or KLF4 siRNAs, were treated with TPA (20 ng/uL) for 48 hours and immunoblot analysis was performed to compare the levels of endogenous KLF4 and lytic viral proteins Z, R and BMRF1. Tubulin served as a loading control.