Fig 7. KLF4 synergizes with BLIMP1 to activate both Rp and Zp, irrespective of the promoter methylation state.
A) Mock treated or methylated promoterless (empty) and Rp-673 pCpGL luciferase constructs were co-transfected into EBV-negative NOKs cells with either control vector, KLF4 vector alone, BLIMP1 vector alone, or the combination of KLF4 and BLIMP1 vectors. Luciferase activity for each of the conditions is shown; values are given as average +/- the standard deviations of results from two replicates. B) Mock treated or methylated promoterless vector and Zp-668 pCpGL luciferase constructs were co-transfected into EBV-negative NOKs cells with control vector, KLF4 vector, BLIMP1 vector or KLF4 and BLIMP1. Luciferase activity for each of the conditions is shown; values are given as average +/- the standard deviations of results from two replicates.
