Table 2.
Expression levels of progesterone metabolizing enzymes (standardized against 18S mRNA) in tumorous and non-tumorous (Normal) resected breast tissues from 11 patients (described in Table 1).
| Patient No. | 5αR1 (SRD5A1) a | 5αR2 (SRD5A2) b | 20α-HSO (AKR1C1) a | 3α-HSO-3 (AKR1C2) c | 3α-HSO-2 (AKR1C3) a | |||||
| Normal | Tumor | Normal | Tumor | Normal | Tumor | Normal | Tumor | Normal | Tumor | |
| 1 | 1.68 ± 0.06 | 5.51 ± 0.30 | 0.99 ± 0.02 | 1.82 ± 0.10 | 0.76 ± 0.05 | 0.79 ± 0.11 | 0.16 ± 0.02 | 0.1 ± 0.01d | 1.25 ± 0.05 | 1.11 ± 0.09 |
| 2 | 0.20 ± 0.01 | 3.42 ± 0.44 | 0.11 ± 0.02 | 3.49 ± 0.03 | 3.77 ± 0.26 | 0.31 ± 0.03 | 3.24 ± 0.09 | 0.18 ± 0.02 | 3.81 ± 0.13 | 0.49 ± 0.02 |
| 3 | 0.64 ± 0.06 | 1.26 ± 0.08 | 0.48 ± 0.05 | 1.79 ± 0.09 | 4.31 ± 0.28 | 1.12 ± 0.05 | 3.78 ± 0.33 | 0.89 ± 0.05 | 4.01 ± 0.03 | 1.12 ± 0.20 |
| 4 | 0.88 ± 0.12 | 2.68 ± 0.12 | 0.28 ± 0.05 | 0.20 ± 0.02 | 7.41 ± 0.25 | 0.42 ± 0.02 | 7.20 ± 1.09 | 0.12 ± 0.01 | 7.77 ± 0.93 | 0.65 ± 0.07 |
| 5 | 0.38 ± 0.02 | 1.44 ± 0.10 | 0.92 ± 0.16 | 4.06 ± 0.33 | 6.07 ± 0.40 | 0.19 ± 0.03 | 6.94 ± 0.41 | 0.17 ± 0.02 | 6.54 ± 0.28 | 2.99 ± 0.17 |
| 6 | 0.66 ± 0.02 | 5.08 ± 0.12 | 0.94 ± 0.05 | 1.91 ± 0.15 | 6.04 ± 1.07 | 2.42 ± 0.07 | 5.75 ± 0.98 | 2.18 ± 0.10 | 8.31 ± 0.48 | 1.97 ± 0.09 |
| 7 | 0.20 ± 0.01 | 4.40 ± 0.46 | 0.26 ± 0.02 | 2.28 ± 0.12 | 4.79 ± 0.60 | 1.78 ± 0.06 | 6.01 ± 1.09 | 2.39 ± 0.03 | 6.07 ± 0.27 | 2.52 ± 0.15 |
| 8 | 0.58 ± 0.08 | 4.70 ± 0.12 | 0.65 ± 0.10 | 0.77 ± 0.02 | 1.51 ± 0.05 | 1.71 ± 0.13 | 1.89 ± 0.06 | 5.26 ± 0.88 | 2.83 ± 0.35 | 6.05 ± 0.42 |
| 9 | 0.20 ± 0.01 | 2.58 ± 0.12 | 0.38 ± 0.11 | 2.41 ± 0.24 | 4.70 ± 0.83 | 1.50 ± 0.16 | 5.59 ± 0.25 | 0.67 ± 0.14 | 6.63 ± 0.54 | 1.47 ± 0.10 |
| 10 | 1.08 ± 0.28 | 4.32 ± 0.22 | 0.61 ± 0.09 | 2.34 ± 0.14 | 5.50 ± 0.51 | 0.68 ± 0.09 | 4.66 ± 0.42 | 0.72 ± 0.14 | 5.25 ± 0.11 | 1.52 ± 0.14 |
| 11 | 1.32 ± 0.18 | 3.08 ± 0.26 | 1.22 ± 0.15 | 2.46 ± 0.09 | 6.04 ± 0.95 | 0.56 ± 0.07 | 6.32 ± 1.01 | 0.42 ± 0.07 | 5.78 ± 0.10 | 1.04 ± 0.08 |
| Mean (±SEM) | 0.709 (0.147) | 3.496 (0.43) | 0.614 (0.105) | 2.139 (0.326) | 4.627 (0.604) | 1.044 (0.217) | 4.685 (0.667) | 1.191 (0.472) | 5.296 (0.644) | 1.905 (0.473) |
Values were obtained from the average of duplicate PCRs run on the same gel and assays were repeated three times for each gene, using a fresh cDNA sample each time. Values are presented as mean ± SEM, n = 3. a Values were obtained by dividing the calculated intensity of 5αR1 or HSO bands (27 cycles) by the calculated intensity of the 18S band (12 cycles). b Values were obtained by dividing the calculated intensity of 5αR2 bands (33 cycles) by the calculated intensity of the 18S band (12 cycles). c Values obtained from very faint bands, calculated as <0.1, were adjusted to 0.1.