Table 2. Affinity determination by QCM (KD values in nM).
Protein | human | mouse | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
IgG | Fab | Fc | IgG | Fab | Fc | ||||||||
pH | KD1 | KD2 | KD1 | KD2 | KD1 | KD2 | KD1 | KD2 | KD1 | KD2 | KD1 | KD2 | |
scDb-SpGC3 | 7.4 | 3 | 38 | 159 | 663 | 6 | 17 | 33 | 593 | 118 | 1100 | 79 | 926 |
6 | 2 | 33 | 95 | 667 | 1 | 28 | 27 | 406 | 130 | 874 | 53 | 858 | |
scDb-SpGC3Fab | 7.4 | 120 | 983 | 88 | 969 | - | - | 61 | 1030 | 96 | 1620 | - | - |
6 | 169 | 1520 | 83 | 753 | 767 | 896 | 54 | 822 | 93 | 826 | 38 | 414 | |
scDb-SpGC3FabRR | 7.4 | 29 | 385 | 167 | 1040 | - | - | 55 | 388 | 62 | 413 | - | - |
6 | 49 | 191 | 43 | 461 | 39 | 227 | 34 | 296 | 19 | 255 | 40 | 289 |