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AXN1 expression levels were knocked down by transfection of AXN1-specific shRNA into HepJ5 cells as described in the Methods and Materials. (A) The expression levels of AXN1 in control and AXN1-KD cells were determined by real-time PCR and western blotting. (B) The migration of scrambled control and AXN1-KD cells was determined by the transwell migration assay as described in the Methods and Materials.
