Table 5. List of unregulated proteins in the 2DE map of either the well-differentiated HepG2 cells or the poorly differentiated SK-Hep–1 cells identified by MALDI-TOF MS.

Cellular compartemts	Spot No	Association code	Name	Theoretical (kDa)/pI	HepG2	SK-Hep–1	Fold	Cellular localization	Function
Total Cell lysate	1	NP005887	NADP+-dependent isocitrate dehydrogenase	46.944/6.34	↑	↓	0.01	Mitochondria	Metabolic enzyme in TCA cycle
	2	P32119	Peroxiredoxin II	21.918/5.67	↑	↓	0.27	Cytoplasm	Antioxidant enzyme
	3	NP003350	UDP-glucose dehydrogenase	55.674/6.73	↑	↓	0.19	Cytoplasm	Metabolic enzyme in Glycosaminoglycan biosynthesis
	4	P09525	Annexin 4	33.757/5.64	↑	↓	0.34	Mitochondria	Calcium binding protein
	5	P33992	Replication licensing factor MCM5	82.990/8.56	↓	↑	4.09	Nucleus	Initiation of DNA replication
	6	P06753	Tropomyosin3	27.386/4.77	↓	↑	4.1	Cytoplasm	Cytoskeleton
Cytosol	1	P09525	Annexin 4	35.957/5.85	↑	↓	0.45	Mitochondria	Calcium binding protein
	2	NP005887	NADP+-dependent isocitrate dehydrogenase	46.944/6.34	↑	↓	0.01	Mitochondria	Metabolic enzyme in TAC cycle
	3	NP00067	MnSOD	22.304/6.86	↑	↓	0.2	Mitochondria	Antioxidant enzyme
	4	NP859048	Peroxiredoxin I	14.054/6.25	↑	↓	0.22	Mitochondria	Antioxidant enzyme
	5	P32119	Peroxiredoxin II	21.918/5.67	↓	↑	0.6	Cytoplasm	Antioxidant enzyme
	6	NP003350	UDP-glucose dehydrogenase	55.674/6.73	↑	↓	0.34	Cytoplasm	Glycosaminoglycan
	7	P04792	Heat Shock Protein 27	22.427/7.83	↓	↑	1.03	Nucleus/ Cytoplasm	Protein folding
	8	P08670	Vimentin	53.545/5.06	↓	↑	1.23	Cytoplasm	Cell motility
Nucleus	1	P04083	Annexin 1	38.787/6.64	↓	↑	4.53	Plasm membrane	Calcium binding protein
	2	P07355	Annexin 2	38.677/7.56	↓	↑	3.27	Plasm membrane	Calcium binding protein
	3	P33991	Replication Licensing factor,MCM4	97.068/6.28	↓	↑	1.17	Nucleus	Initiation of DNA replication biosynthesis
	4	NP031478	Peroxiredoxin III	28.047/6.73	↑	↓	0.08	Mitochondria	Antioxidant enzyme
Membrane	1	P08670	Vimentin	49.680/5.19	↓	↑	3.57	Cytoplasm	Cell motility
	2	P35232	Prohibitin	29.859/5.57	↑	↓	0.14	Mitochondria	Cell proliferation