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. 2015 Oct 2;9(10):e0004041. doi: 10.1371/journal.pntd.0004041

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© 2015 Firdessa et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — PHMB disrupted membrane integrity and condensed DNA in L. major promastigotes. Representative FACS histogram (a) propidium iodide (PI) and (b) YO-PRO-1dye staining of promastigotes after treatment with 2 μM PHMB for indicated time points, showing time-dependent effect of PHMB on the membrane integrity. Heat-killed promastigotes and amphotericin B at 2 μM concentration were used as positive controls. (c) Fluorescent microscopy analysis showing condensed and damaged DNA materials of L. major promastigotes after treatment with PHMB at 2 μM for 48 h as compared to the mock control (distilled water). Scale bars = 5 μm.