Fig 2. Inhibition of EV-A71 attachment and infection by commercially-available NF449 analogues.

(A) Structures of the tested compounds. (B) ³⁵S-labeled EV-A71-1095 was exposed to compounds at 0.4 or 4 μM, then virus attachment to RD monolayers was measured. (C) RD monolayers were exposed to EV-A71-1095 in the presence of inhibitors at the indicated concentrations. After 16 hrs at 37°C, infected cells were stained to detect intracellular VP1, and analyzed by flow cytometry. Results are indicated as the mean and S.D. of triplicate samples. Asterisks indicate P < 0.01 compared to the no inhibitor control.