Figure 4.

Dual labeling of aquaporin 1 and N-cadherin in the outer cortex. Samples were processed for the dual labeling of the proximal tubule marker aquaporin 1 and N-cadherin as described in the Methods and then viewed using a 40× or a 100× objective. Panels A-D show lower power images of the same field. A: Aquaporin 1; B: N-cadherin; C: Overlay of images A and B; D: Phase contrast image of the same field. Panels E and F show the dual labeling in a different field viewed at higher magnification. E: Aquaporin 1; F: N-cadherin. Original magnification = 174× (Panels A-D) and 435× (Panels E and F).