Fig. 5.

Quantitative real-time PCR analysis of venom allergen 5 at different developmental stages of Cx. pipiens pallens. The venom allergen 5 gene was transcribed at all developmental stages. The transcriptional levels of venom allergen 5 were 2.2-, 3.3-, and 12.3-fold higher in the DR1 strain than in the DS strain at the egg, female, and male adult stages. The expression level of mosquitoes at the egg stage of the DS strain was considered to be 1. The figures show the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 compared with the DS strain.