Table 2.
Fragment | Sense primer (5′ to 3′) | Antisense primer (5′ to 3′) |
---|---|---|
ORF | ATGTCGATCAACCGTAAAGTTC | TCAAGCTCCCACATCCACCG |
5′-RACE | Universal Primer A Mixa | CGCATCCACGCAATCCGACGACAGC |
3′-RACE | CACTTTGCCACGCCGAAGCTGATGA | Universal Primer A Mixa |
aThe Universal Primer A Mix (UPM) was a mixture of 5′-CTAATACGACTCACTATAGGGC AAGCAGTGGTATCAACGCAGAGT-3′ and 5′-CTAATACGACTCACTATAGGGC-3′.