Figure 1. Expression of FGFR1 protein in HNSCC cell lines.
A, Extracts from the indicated HNSCC cell lines were submitted to SDS-PAGE and immunoblotted for FGFR1 with an antibody against the C-terminus and the α-subunit of NaK-ATPase as a loading control. B, Extracts from 584-A2 and CCL30 were submitted to immunoblot analysis using an antibody recognizing an epitope within the extracellular domain. Note that the FGFR1 polypeptide in CCL30 cells is not detected by the C-terminal-directed antibody, suggesting that the cells express an alternatively spliced variant.