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Determination of post-transplant T cell phenotype. CD4+ T cells were evaluated by intra-cellular flow cytometry for expression of transcription factors for Th2, Th1, or regulatory T cell subsets (GATA-3, left panel; T-bet, middle panel; FoxP3, right panel). Time points evaluated were before initiation of the PC regimen (day -21) and at the indicated post-transplant times.
