VR-SIM images and subsequent H&E slide images of biopsy B18 classified as non-malignant, but containing high-grade prostatic intraepithelial neoplasia (HGPIN). A) VR-SIM mosaic image of the entire biopsy, comprising 205.5 megapixels, and B) Digital image of the corresponding H&E section. Zooms of the dashed yellow boxes in A and B are shown in C and D, respectively. C) A close-up of a gland containing PIN (yellow arrow) in the VR-SIM image, with D) the corresponding H&E image.