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. Author manuscript; available in PMC: 2015 Oct 6.
Published in final edited form as: Leuk Lymphoma. 2015 Jan 21;56(8):2439–2447. doi: 10.3109/10428194.2014.996751

Figure 4.

Figure 4

Knockdown of ABL1 or TP73 in MEC1 cells. (A) Protein levels of ABL1 and TAp73 analyzed by Western blot upon 48 h of treatment with 10 μM CDDP as indicated after lentiviral transduction of MEC1 cells with a shRNA targeting ABL1 or TP73 (two constructs) or a non-targeting shRNA. (B) mRNA expression levels, assessed by qRT-PCR, of CDKN1A, PUMA and BID upon treatment of lentiviral transduced MEC1 cells with CDDP 10 μM for 48 h. Plotted is fold induction compared to untreated cells; dotted line represents “no induction” (fold induction = 1). (C) Apoptosis after 48 h of treatment with CDDP as indicated, assessed by MitoTracker staining; mean ± SEM of three experiments. MEC-NO = MEC1 cell transduced with nonsense shRNA; *p < 0.05 (paired t-test).