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. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Anal Chem. 2015 Oct 6;87(19):9671–9678. doi: 10.1021/acs.analchem.5b01663

Figure 2.

Figure 2

MS/MS spectra of the abundant peptides from bovine fetuin serum. Fetuin peptides are digested using trypsin via (A) in solution and (B) iGIG. Peptides that contain E or D, such as HTFSGVASVESSSGEAFHVGK, can be modified by p-toluidine on iGIG; thereby, the retention time differs between the native (42.27 min) and the modified peptide (65.68 min).