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. 2015 Oct 3;12:34. doi: 10.1186/s12981-015-0066-7

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© Nankya et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Neighbor joining phylogenic trees of two dual tropic HIV-1 isolates, two HIV-1 env chimeric viruses, and of the sampled clones in their virus population. Alignments and phylogenetic trees were constructed for the C2-V3 region (350 nt) of the four reference strains, r5 B2, r5 C3, x4 A8, and x4 E6, the two dual tropic HIV-1 isolates (C19 and C27), the two HIV-1 env chimeric viruses (B12 and B19) and set of reference strains (a). As described in the “Methods”, the Env coding region of two dual/mixed (dm) HIV-1 isolates were PCR amplified and cloned into pREC env via yeast-based recombination. The C2-V3 sequences from 21 B19 (b), 18 B19 (c), 26 C19 (d), 21 © clones (e) were aligned using MUSCLE and then schematic represented in phylogenetic trees.