Table 1.
Glycanase activities from the larval digestive tract of the T. dichotomus.
| Organ | Substrate | Optimum pH | Total Activity (mU) | Specific Activity | |
|---|---|---|---|---|---|
| (mU/mg Protein) | (mU/g Organ) | ||||
| Foregut | CM-cellulose | ND a | 188 b ± 25 c | 4.79 ± 0.55 | 164 ± 23 |
| β-1,3-glucan | 6.15 | 194 ± 24 | 4.95 ± 0.69 | 169 ± 26 | |
| β-1,4-xylan | 7.63 | 1733 ± 415 | 44.25 ± 11.37 | 1514 ± 9 | |
| Pectin | 6.11 | 2540 ± 360 | 64.86 ± 8.20 | 2219 ± 8 | |
| Soluble starch | 8.66 | 127 ± 26 | 3.23 ± 0.63 | 111 ± 19 | |
| Midgut | CM-cellulose | ND a | 319 ± 28 | 8.11 ± 0.77 | 52 ± 6 |
| β-1,3-glucan | 6.22 | 3066 ± 220 | 77.95 ± 4.15 | 502 ± 36 | |
| β-1,4-xylan | 6.18, 7.69 | 7094 ± 543 | 180.36 ± 16.14 | 1162 ± 3 | |
| Pectin | 8.53 | 2982 ± 261 | 75.80 ± 7.18 | 488 ± 1 | |
| Soluble starch | 7.07 | 13,520 ± 569 | 343.74 ± 13.19 | 2214 ± 85 | |
| Hindgut | CM-cellulose | ND a | 80 ± 14 | 5.85 ± 1.02 | 16 ± 4 |
| β-1,3-glucan | 6.14 | 856 ± 87 | 62.63 ± 6.94 | 170 ± 20 | |
| β-1,4-xylan | 6.98 | 2039 ± 311 | 149.19 ± 27.82 | 404 ± 8 | |
| Pectin | 6.76 | 2836 ± 105 | 207.51 ± 13.08 | 562 ± 4 | |
| Soluble starch | 8.69 | 145 ± 36 | 10.61 ± 3.02 | 29 ± 8 | |
a Not detected; b total activity for the carboxymethyl cellulose (CMC) degradation was measured using GTA buffer at pH 7.0; c SE, standard error (n = 6); the organ weight used is as follows: foregut, 0.7759–1.8733 g; midgut, 3.7972–8.7650 g; hindgut, 3.1010–7.0759 g.