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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Proteins. 2015 May 8;83(7):1225–1237. doi: 10.1002/prot.24699

Figure 3.

Figure 3

Some optical and biophysical properties of eCGP123 and TGP.

(A) Fluorescence images of eCGP123 and TGP solutions each at 1.5 mg/ml after an overnight dialysis against PBS at 4°C. The cassette containing eCGP123 (left) is precipitating whereas TGP (right) is not. (B) Centrifugation of the samples in panel (A), shows that nearly all of the eCGP123 has precipitated out of solution. (C) The time dependent changes in fluorescence emission of TGP, eCGP123, and mAG on incubation at 85°C or 90°C in buffer consisting of 50mM Tris pH 7.5, 150mM NaCl, 10% Glycerol, 2mM EDTA. (E) The excitation and emission spectra (D) and molar absorptivities (E) of TGP, eCGP123 and mAG are shown.