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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Alzheimers Dement. 2015 Apr 4;11(11):1286–1305. doi: 10.1016/j.jalz.2015.01.005

Fig.6. High and Intermediate mwt oligomers are detected by ELISA in aqueous extracts of AD brain fractionated on SEC in physiological buffer.

Fig.6

TBS extract (1 ml) from AD brain 30 was chromatographed on a Superdex 75 10/30 HR column eluted in 50 mM ammonium acetate (A and C) or artificial CSF (aCSF) (B and D). Half ml fractions were collected and analyzed using the oligomer-specific ELISA (oELISA) (A and B) or the Aβx-42 ELISA (C and D). High/intermediate mwt oligomers were detected in fxs 1–4, whereas the majority of Aβx-42 was found in fxs 14–18. Oligomer concentrations were calculated using an ADDL standard with units of equivalent weight of Aβ1-42 monomer (see Yang et al. 2015) and Aβx-42 concentration were calculated based on the molar content of SEC-isolated Aβ1-42 monomer. Fraction “0” was not examined by ELISA, and ND indicates that the concentration was not determined.