(A–D) Foxj1 was stained in uninjected control (uninj.) embryos (A), as well as manipulated embryos (injected side shown in B–D). (B) Inhibition of Notch by injection of Su(H)-DBM mRNA increased foxj1 expression, which remained dependent on ATP4a (C) and Wnt/β-catenin (β-cat.; D). (E) Quantification of results. Staining intensity on the injected (right) side was compared to the uninjected (left) control side and quantified as right/injected side stronger, weaker or equal to the control side (G).
a, anterior; d, dorsal; n, number of embryos; ns, not significant; p, posterior; st., stage; v, ventral.