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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Dev Biol. 2015 Apr 4;408(2):292–304. doi: 10.1016/j.ydbio.2015.03.013

Fig. 5. Serotonin secretion and tph1 expression in the embryonic epidermis are regulated by ATP4a-mediated Wnt/β-catenin signaling.

Fig. 5

(A–D) Immunofluorescent analysis and quantification (E) of serotonin (5-HT; red) deposition in small secretory cells (SSCs) of the skin at stage 32. Actin (phalloidin-Alexa488) staining in green. (A) Uninjected (uninj.) control. (B) Loss of serotonin staining in atp4a morphants. (C, D) Rescue of serotonin staining upon co-injection of atp4a (C) or β-catenin (β-cat.; D) DNA constructs. (A′–D′) Serotonin channel. (A″–D″) Higher magnification of (A–D). (E) Quantification of results. (F–H) WMISH for tph1. (F, F′) Uninjected control. (G, G′) Loss of tph1 expression in atp4a morphants was rescued by co-injection of atp4a DNA (H).

n, number of embryos; st., stage.