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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: J Immunol. 2015 Sep 2;195(8):3557–3564. doi: 10.4049/jimmunol.1501407

FIGURE 4. Comparison between CD4+ and CD8+ 3.L2 T cells for TCR–pMHC-II binding.

FIGURE 4

(A) Flow cytometry scatter plot of 3.L2 T-cells. CD4+ and CD8+ cells were purified with respective CD4 and CD8 negative purification protocols then analyzed for TCR, CD4, and CD8 expressions by flow cytometry. (B) Comparison of effective 2D affinity between CD4+ and CD8+ 3.L2 T cells. No significant difference were observed between most of the two subpopulation. Student's t-test was used for statistical analysis. Each bar represents mean ± SEM (n≥3).