Figure 3. Effect of LTC4S deletion on IL-33 expression and attendant physiologic effects.
A. BAL fluid total cell counts and eosinophil counts in the lungs of ptges−/− and ptges−/−/ltc4s−/− mice measured 24 h after the last of 6 doses of PBS or Df. B. ELISA of total IL-33 protein levels in lung lysates from the indicated strains. C. Western blotting of lung proteins from the indicated strains showing full-length and cleaved forms of IL-33. D. Quantitative densitometry showing effect of ltc4s deletion from ptges−/− mice on lung IL-33 protein levels. E. BAL fluid levels of MC granule-derived mediators from the indicated groups. Results in A, B, and D and E are mean ± SEM from at least 10 mice/group in two separate experiments. * p<0.05, ** p<0.01, *** p<0.001.