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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: J Immunol. 2015 Sep 14;195(8):3525–3529. doi: 10.4049/jimmunol.1500436

FIGURE 3. IL-25-induced inflammation is ameliorated in CD11c Δ CIKS mice.

FIGURE 3

(A) Relative expression of Ccl17, Il9, Il13 and Il5 in total lung cells of WT and CD11c Δ CIKS mice, 24h after 3 daily challenges with IL-25 or PBS. (B) Numbers of basophils (DX-5+ FcRεI+ c-Kit-), mast cells (DX-5- FcRεI+ c-Kit+) and ILC2s (Lin- St2+ Icos+) in total lung cells isolated from challenged mice as in (A). (C) Numbers of CD4+ TCRβ+ T cells in BAL fluid or total lung isolated from mice as in (A) after 4 daily challenges with IL-25 or PBS (D) Numbers of IL-9+ CD4+TCRβ+ T and ‘non-T’ cells (CD4- TCRβ-) in total lung and protein levels of IL-9 in BAL fluid and CCL17 in total lung in challenged mice as in (C). (E) CD4+TCRβ+ T cell numbers and IL-9 protein levels in BAL fluid and relative expression of Il9 and Il13 in total lung of WT and CCL17 KO mice challenged as in (A). Data shown as mean ± sem, based on three (A–D) or two (E) independent experiments with n=7–9 or n=6 mice/genotype/condition, respectively. * p < 0.05.