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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: J Immunol. 2015 Sep 4;195(8):3946–3958. doi: 10.4049/jimmunol.1500966

Figure 2. Selectivity and binding profiles of the anti-Efb-C miniAbs generated from a human F(ab)2 library screening.

Figure 2

(A) Left Panel. Relative binding activities of the miniAbs towards selected complement modulatory proteins of S. aureus as determined by SPR analysis. RU, resonance units. Right Panel. Schematic outline of the SPR-based experimental setup employed to determine the selectivity of the various miniAbs. (B) Kinetic analysis of the interaction between selected miniAbs and Efb-C. The processed binding curves (black lines) were fitted to kinetic models (red lines). The KD values corresponding to each miniAb are shown in the respective sensogram. Data are representative of three independent experiments. (C) Immunoreactivity of selected miniAbs with i) a group of selected S. aureus recombinant proteins or ii) culture supernatants from different S. aureus strains (Newman, ΔEfb, ΔEhp). Immunoblot analysis using a polyclonal antiserum raised against Efb-C was performed in parallel to ensure protein presence and integrity.