TABLE 1.
B. subtilis strains and plasmids used in this study
| Strain or plasmid | Genotype, phenotype, or description | Source or referencea |
|---|---|---|
| Strains | ||
| GP45 | trpC2 rnjB::spc Spcr | J. Stülke |
| WN624 | trpC2 amyE::spc Spcr, ancestral strain | 17 |
| WN628 | trpC2 amyE::cat Cmr, ancestral strain | 17 |
| WN1106 | trpC2 amyE::spc Spcr, evolved to enhanced growth at 5 kPa | 8 |
| WN1261 | trpC2 amyE::neo, Neor in WN624 background | pECE141→WN624 (tf); this study |
| WN1278 | trpC2, amyE::neo, Neor in WN1106 background | pECE141→WN1106 (tf); this study |
| WN1279 | trpC2 amyE::cat, Cmr in WN1106 background | pDAG32→WN1106 (tf); this study |
| WN1518 | trpC2 amyE::neo rnjB::spc, Neor Spcr in WN624 background | GP45→WN1261 (tf); this study |
| WN1519 | trpC2 amyE::neo rnjB::spc, Neor Spcr in WN1106 background | GP45→WN1278 (tf); this study |
| 50–1,000 generations | amyE::spc Spcr; 20 frozen stock populations representing generations 50 to 1,000 of the 5-kPa evolution expt | 8 |
| Plasmids | ||
| pECE73 | pCm::Neo antibiotic switching cassette | BGSC (16) |
| pECE141 | pSpc::Neo antibiotic switching cassette | BGSC (16) |
| pDAG32 | pSpc::Cm antibiotic switching cassette | BGSC (16) |
a
Abbreviations: tf, transformation; BGSC, Bacillus Genetic Stock Center.