TABLE 2.
Oligonucleotide primers used in this study
| Primer type | Olignucleotide | Sequence (5′→3′) |
|---|---|---|
| WN624a | CITZF230 | GGGGAAATCACAGAATGGAA |
| CITZR1225 | ATGTACGGCTCAGCGTTTTC | |
| COMPF1673 | TGAACGAGGCGGTAGTTCTT | |
| COMPR2285 | GTGCTTGACCGCATTAGACA | |
| EPSCF686 | TGACGACCAAACGAAGCATA | |
| EPSCR1311 | TGTTTTTGACCGCCTCTTCT | |
| GERAF855 | TCACCATTTCCGCAGATACA | |
| GERAR1584 | TTGGGAGACGGATAATGGAG | |
| GLTAF2805 | ATGTCATTCGGGTCCTTGAG | |
| GLTAR3774 | TTGCTTTCCAGTGCTCCTTT | |
| ILVCF106 | ACAGGTACAACGGCTTTTGC | |
| ILVCF366 | GCCCAAGAAGACGGACATAA | |
| ILVCR1136 | TTGATGTTCGTTCTCGCTTG | |
| ILVCR1186 | TGTTTCACAAACGGCATCAT | |
| OPPDF446 | GCTGTCCGAAAAAGAAATGC | |
| OPPDR1409 | GATTCACCAACCAGCCCTAA | |
| PGDSF206 | CACACTGGCAAACTGGAAGA | |
| PGDSR1164 | CAAATCCCGTCTCAGGTGTT | |
| RECAF183 | AGCCTGGGCATGTGTTTATC | |
| RECAR1106 | CCCGAACATAACACCGACTT | |
| RLUBF608 | GAAAGGAATCCCGCCTAAAG | |
| RLUBR1198 | GCTCGATACGTTTCCCATTC | |
| RLUDF173 | AGCGGGAAAAAGAAAAAGGA | |
| RLUDR1150 | TTCAGCTTCGTTTATTATTTCAGA | |
| MSWCF372 | GGAGAGCATTCCTGTTTTCG | |
| MSWCR1079 | CGCCATTTCCGTATCAGAAT | |
| SACAF1339 | CGGAGTCTGGCTTTTCAATC | |
| SACAR1993 | CGGCAGCCTGTTTATTTGAT | |
| SCOCF82 | AAAAACCCTTTCGTCGCAAT | |
| SCOCR1037 | GAGCAAGGAAGAAGGGGAAG | |
| SEPFF133 | GCTGAGAGAACTCCGTGACC | |
| SEPFR940 | TCGGAGGGATGATTTTTCTG | |
| SFTAF480 | AGAGAGCGTCCCAGAAATGA | |
| SFTAR1454 | AGACGATTTGGAACCCTGCT | |
| SIGIF137 | TTCTCCTTTGCGAATCCCTA | |
| SIGIR1004 | TCAGCAACGTGACGAATTTT | |
| SSRBF268 | GAATCGCACTCGGCTTAGAC | |
| SSRBR1008 | TGTCCGACAGTTGAAAGCAG | |
| TRMDF262 | TTCTTCAAAAAGCCCAGGAA | |
| TRMDR1147 | AAGTGTCACCAGGACGGAAC | |
| UVRXF275 | GGCTGTAACAATGGGGCTTA | |
| UVRXR951 | GCATCAACGGAGCACCTATT | |
| VEGF160 | GCTTTACGCCGTTTATGGAA | |
| VEGR804 | AAAGGGCAAAACAAAAAGCA | |
| YOJAR937 | TGTTGCCATTGCCATAAAGA | |
| YOQAF81 | CGGAGTGACAAGTGAAATGC | |
| YOZTF187 | TTGGATGTCCCGATGAAAAT | |
| YOZTR842 | CTTGAGAGCGGGATGGTAAT | |
| YPIBF97 | ATTTTGCGAATCGAGGATGA | |
| YPIBR806 | TCTCCACCTCATCGTTCTCC | |
| YQEZF354 | CGTTCGTTACAAGACGCAAA | |
| YQEZR1217 | AGCTGAGGCGATCAAGAGAG | |
| YUTEF14 | TTGTGGGAGACTGGGGATAC | |
| YUTER749 | ACAAATTCACACGCCTCCTC | |
| YXBDF121 | TGACCCCAGAGCAAATCTTC | |
| YXBDR712 | TTTCGGGCTTCATCTTTCTG | |
| YXJMF412 | TCCTGACCGATTTCTTTTGG | |
| YXJMR919 | TCTGAATCCTCCTTGCTGCT | |
| WN1106b | bacD-F3871584 | AGAGCAGCACGGAAATCTTCA |
| bacD-R3872064 | TCATCGCTGATCTTGGAGGC | |
| fliI-F1695745 | TGCTGATGAAAAAGCTCAAAAAGG | |
| fliI-R1696207 | GCGGTTCTCCAAAAGCATCG | |
| parC-F1935866 | ACACGGTTGAATTTGTGCCG | |
| parC-R1936322 | TCACGAACCTCTGAGATGCC | |
| resD-F2417342 | GAAGCTTTTCCCGATCATACACC | |
| resD-R2417791 | ATGGTGATGAAGCCATTGCC | |
| rnjB+261F | AACAAGCTGTCCGTTCCAGT | |
| rnjB+771R | TTCCGGCTACGGCAATCTTT | |
| walK-F4152887 | CAGAGGCTAGCTTTCTGCGT | |
| walK-R4153352 | GTGGCTGGGAAACAAACGAC | |
| yto-F2998178 | TCATCCGTGTTAAAGCCCCC | |
| yto-R2998587 | ATCGATTCACTGCCTGTCGG | |
| yvlD-F3606521 | GCACAACAGAGGGAGTGCAA | |
| yvlD-R3607016 | GCCAGCCTCATTTTATCGATCTT |
a
Primers used for PCR amplification and confirmation by Sanger sequencing of the SNP-containing regions found in strain WN624, which differed from the strain 168 sequence (see Table S1 in the supplemental material).
b
Primers used to verify SNPs identified in strain WN1106 (see Table 3).