Box-and-whisker plot (top) and temporal profile (bottom) of the dsrB copy numbers compared with the T-M mcrA copy numbers estimated in the same sample. Data in the temporal profile are presented in order of sampling: from the left, April 2010, August 2011, and January, August, and December 2012 for WCA-2A sites F1, F4, and U3 and February 2012 and March and April 2013 for site W3. Error bars in the bar graph represent ±1 SE (n = 3). The box-and-whisker plot was constructed from the pooled data from the temporal profile. Boxes depict the medians (horizontal lines in the boxes) and the lower and upper quartiles (bottoms and tops of the boxes, respectively), while the whiskers show the highest and the lowest values, excluding outliers. The different letters indicate a significant difference between dsrB and T-M mcrA copy numbers (P < 0.05 by Student's t test).