FIG 3.
Influence of pH on anaerobic growth of C. glutamicum in a bioreactor containing MOPS-free CGXII minimal medium with 4% (wt/vol) glucose and either no tryptone (A and B) or 15 g liter−1 tryptone (C and D). The following pH values were tested: 5.5 (○), 6.0 (□), 6.5 (△), 7.0 (■), 7.5 (●), 8.0 (▲), and 8.5 (◆). The bioreactor was flushed with pure N2 at 0.3 liter min−1. The pH was kept constant by addition of 3 M KOH or 3 M HCl. Average values for at least two independent replicates are shown.