FIG 7.

Effect of riboflavin amount in cultivation step on lumichrome production by resting cell reaction. (A) Microbacterium sp. strain TPU 3598. (B) Devosia riboflavina ATCC 9526. Cells were cultivated in 10 ml of the nutrient medium at 30°C for 16 h. Lumichrome production by resting cell reaction was carried out with 200 mg of cells for 8 h in 10 ml of 10 mM KPB, pH 7.0, containing 50 μmol riboflavin. Black and white bars correspond to the amounts of lumichrome and riboflavin, respectively. Error bars indicate the standard deviations obtained from three replicates.