FIG 1.

Locations where B. burgdorferi samples that have been subjected to MLST analysis have been collected in North America. The colored points indicate locations where all samples analyzed were collected, while red arrows indicate the locations of field sites where new samples in a recent study in Canada were obtained (49). The different colored points correspond to sequence types (STs) found in different geographic regions. Cyan, STs found only in the Maritimes, Canada; orange, STs found only at Long Point, Ontario, Canada; brown, STs found only in Manitoba, Canada; blue, STs found across the northeastern United States and southern Quebec, southeastern Ontario, and the Maritimes in Canada; green, STs found in the midwestern United States; yellow, STs found in both northeastern and midwestern locations of the United States and longitudinally corresponding regions of Canada; red, STs found only in California. The dashed black arrows indicate the likely locations of immediate ancestors of novel STs found in Canada. Maps were created in ArcGIS. (Adapted from reference 49.)