TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference |
|---|---|---|
| Strains | ||
| Ralstonia pseudosolanacearum | ||
| Ps29 | Wild-type strain; race 1, biovar 3, phylotype I | 23 |
| DPS01 | Ps29 derivative; ΔmcpA | This study |
| DPS14 | Ps29 derivative; ΔmcpM | This study |
| MAFF106611 | Wild type; race 1, biovar 4, phylotype I | 23 |
| DMF01 | MAFF106611 derivative; ΔmcpA | This study |
| DMF14 | MAFF106611 derivative; ΔmcpM | This study |
| DMFcheA | MAFF106611 derivative; ΔcheA | This study |
| MFK | MAFF106611 derivative; Kmr | This study |
| Escherichia coli | ||
| JM109 | recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) e14 negative (mcrA negative) supE44 relA1 Δ(lac-proAB) F′ [traD36 proAB+ lacIq lacZΔM15] | 24 |
| S17-1 | MM294 derivative; RP4-2 Tc::Mu-Km::Tn7; chromosomally integrated | 25 |
| Plasmids | ||
| pK18mobsacB | Kmr pUC18 derivative; lacZα mobs site sacB | 26 |
| pNMPS01 | pK18mobsacB with a 1.2-kb PCR fragment upstream of mcpA and a 1.1-kb PCR fragment downstream of mcpA from the Ps29 genome; Kmr | |
| pNMPS14 | pK18mobsacB with a 0.9-kb PCR fragment upstream of mcpM and a 0.6-kb PCR fragment downstream of mcpM from the Ps29 genome; Kmr | This study |
| pNMMF01 | pK18mobsacB with a 1.2-kb PCR fragment upstream of mcpA and a 1.1-kb PCR fragment downstream of mcpA from the MAFF106611 genome; Kmr | This study |
| pNMMF14 | pK18mobsacB with a 0.9-kb PCR fragment upstream of mcpM and a 0.6-kb PCR fragment downstream of mcpM from the MAFF106611 genome; Kmr | This study |
| pNMMFcheA | pK18mobsacB with a 0.8-kb PCR fragment upstream of cheA and a 0.9-kb PCR fragment downstream of cheA from the MAFF106611 genome; Kmr | This study |
| pTBS | pK18mobsacB with 0.8-kb and 1.2-kb PCR fragments from the MAFF106611 genome; Kmr | This study |
| pINkanR | pTBS with a 1.0-kb fragment including the kanamycin resistance gene; Kmr | This study |
| pUCP18 | E. coli-Pseudomonas shuttle vector derived from pUC18; lac promoter lacZ Cbr | 27 |
| pKZ27 | Broad-host-range transcriptional fusion vector; IncQ lacZ Kmr | 28 |
| pRCII | E. coli-Ralstonia shuttle vector derived from pKZ27; IncQ lac promoter Kmr | This study |
| pPS01 | pRCII with a 2.1-kb PCR fragment including mcpA in Ps29 | This study |
| pPS14 | pRCII with a 2.0-kb PCR fragment including mcpM in Ps29 | This study |
a
Kmr, kanamycin resistance; Cbr, carbenicillin resistance.