TABLE 1.
Primer name | Target group | Sequence (5′-3′) | Adapterb |
---|---|---|---|
Illumina-Ba9F | Bacteria | GAGTTTGATCMTGGCTCAG | A |
Illumina-Ba515Rmod1 | Bacteria | CCGCGGCKGCTGGCAC | B |
Illumina-Ar915aF | Archaea | AGGAATTGGCGGGGGAGCAC | B |
Illumina-Ar1386R | Archaea | GCGGTGTGTGCAAGGAGC | A |
Illumina-RP841F | Rumen ciliates | GACTAGGGATTGGARTGG | B |
Illumina-Reg1302R | Rumen ciliates | AATTGCAAAGATCTATCCC | A |
Illumina-MN100F | Anaerobic fungi | TCCTACCCTTTGTGAATTTG | B |
Illumina-MNGM2 | Anaerobic fungi | CTGCGTTCTTCATCGTTGCG | A |
According to the work of Kittelmann et al. (22).
Primers had Illumina adapter A (5′-TCG TCG GCA GCG TCA GAT GTG AT AAG AGA CAG-3′) or B (5′-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA G-3′) attached to the 5′ end.