TABLE 2.
Primers used in this study
| Primer name | Sequence (5′→3′) | Use |
|---|---|---|
| flhA+ | TATTCGCAGCATTGCAGAGG | flhA-flhF intergenic region amplification |
| flhF− | GCATCGAGTCAAAGGCACGC | flhA-flhF intergenic region amplification |
| flhF+ | CAGTATGGACAGTTTCCGCATCGGC | flhF-fleN intergenic region amplification |
| fleN− | TTGTCGCCAATCTCGCTGAACGCC | flhF-fleN intergenic region amplification |
| fleN+RT | AGGCGTTCAGCGAGATTGGCGACA | fleN-fliA intergenic region amplification |
| fliA−RT | ACGCTCGCGCTCTGGCAAATTGG | fleN-fliA intergenic region amplification |
| fleQ-F | GCGAGAGGTTCTGTGCGTGCTGGTC | qRT-PCR expression of fleQ |
| fleQ-R | GCAGCTCGACGGAAGAGTTTTCGCTCA | |
| fleS-F | CGCCAGTCATCTGACCGAGCAGGAA | qRT-PCR expression of fleS |
| fleS-R | GGGCGTTAGGCGGTCCGTCAGC | |
| fleN-F | AGGCGTTCAGCGAGATTGGCGA | qRT-PCR expression of fleN |
| fleN-R | TCGCACACCACCAGCAGCACTTCCT | |
| fliA-F | CGAGGACGGCGCAAGCGGTTC | qRT-PCR expression of fliA |
| fliA-R | ACGCTCGCGCTCTGGCAAATTGG | |
| flgM-F | CACGTACCGCAGCCAGCAAAGACACC | qRT-PCR expression of flgM |
| flgM-R | TAACGGTAGGCAGGTCGGTCAGCTTGTCC | |
| flgF-F | TCGACCAATGGCTTCATGCGTGACCTT | qRT-PCR expression of flgF |
| flgF-R | TCGGGAGTCTGCACGGCAATCCAG | |
| flhA-F | CGTCGTGCTGCTGGTTTGCGTCTAT | qRT-PCR expression of flhA |
| flhA-R | GCATGACCGTCCTGACCGTGAAGC | |
| flhF-F | GCCGCCACGCCCGTTTGC | qRT-PCR expression of flhF |
| flhF-R | GCTGCGGACGGCTGCCTTGT | |
| fliC-F | AAGGCGCACTGCAAGAGTCGACCAAC | qRT-PCR expression of fliC |
| fliC-R | GGTGCTGGCGGAACCGTCAAGC | |
| syfA-F | CGCACGACGCAACGCAAGGAATGG | qRT-PCR expression of syfA |
| syfA-R | ACGCACGGCACTTACCTGGGCAAA | |
| syfR-F | CCGCTGGTCGAACATCACGCACAA | qRT-PCR expression of syfR |
| syfR-R | AGCCACGGTTCCGCCTGTCAA | |
| gyrA-F | GGCAAGGTCACCCGCTTCAAGGAAT | qRT-PCR expression of gyrA |
| gyrA-R | GACCGCCACGCTTGTACTCAGGGAAC |