FIG 2.

Comparison of chondroitin sulfate A digestion activities among Flavobacterium columnare wild-type, mutant, and complemented strains. (A) Chondroitin lyase activity can be seen as a clear zone around bacterial colonies against a cloudy background. (B) The chondroitin lyase activity of each strain was determined by measuring the amount of residual chondroitin sulfate A after incubation of this compound with the strains for 30 min in a 96-well flat-bottom microplate. The values are means and standard deviations (SD) (n = 3). Asterisks indicate significant differences (P < 0.05).