Table 2.

Substrate specificity, activity of PeiW or PeiP on synthetic peptide substrates using an agarose plate assay. The intensity of a zone of yellow colouration was compared; − no zone, + zone being only just observable, and +++++ very large zone. No yellow zones were observed with control buffer in the wells.

Synthetic peptide substrate	Abbreviation	Relative activity
		PeiW	PeiP
L-Ala-pNA	ApNA	+	+
H-Glu-Ala-pNA	EApNA	+++	++
Glu-γ-Ala-pNA (Glu(Ala-pNA)-OH)	EγApNA	+++++	+++++
Glu-γ-Ser-pNA (Glu(Ser-pNA)-OH)	EγSpNA	+++	++
Glu-γ-Thr-pNA (Glu(Thr-pNA)-OH)	EγTpNA	−	−
Asp-β-Ala-pNA (Asp(Ala-pNA)-OH)	DβApNA	+	+
L-Glu-γ-pNA (Glu(pNA)-OH)	EγpNA	+	−