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. 2015 Oct 5;5:14700. doi: 10.1038/srep14700

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Copyright © 2015, Macmillan Publishers Limited

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(a) Averaged indentation curves for PIP₂ microinjected (red) and control cells (black). (b,c) Overall tension . (d,e) Apparent area compressibility modulus . (b, d) Histograms obtained from analysis of force indentation curves on control cells (grey) and 3 h after injection of PIP₂ (red). (c,e) show and as a function of elapsed time after PIP₂ injection. Box plots extend from the 30th to the 70th percentile, whiskers from the 20th to the 80th. Grey bars in the histograms and box plots refer to data obtained from untreated cells, red ones represent PIP₂ microinjected cells. (b,c) n = 196 (control), 132 (2 h), 106 (3 h), 101 (4 h), 71 (5 h) analysed force distance curves. (d,e) n = 196 (control), 132 (2 h), 102 (3 h), 101 (4 h), 71 (5 h) analysed force distance curves. n > 5 (control), n = 3 (PIP₂) analysed cells. Asterisks indicate a statistical difference (***p < 0.001, Wilcoxon rank sum test).

Inline graphic — (a) Averaged indentation curves for PIP₂ microinjected (red) and control cells (black). (b,c) Overall tension . (d,e) Apparent area compressibility modulus . (b, d) Histograms obtained from analysis of force indentation curves on control cells (grey) and 3 h after injection of PIP₂ (red). (c,e) show and as a function of elapsed time after PIP₂ injection. Box plots extend from the 30th to the 70th percentile, whiskers from the 20th to the 80th. Grey bars in the histograms and box plots refer to data obtained from untreated cells, red ones represent PIP₂ microinjected cells. (b,c) n = 196 (control), 132 (2 h), 106 (3 h), 101 (4 h), 71 (5 h) analysed force distance curves. (d,e) n = 196 (control), 132 (2 h), 102 (3 h), 101 (4 h), 71 (5 h) analysed force distance curves. n > 5 (control), n = 3 (PIP₂) analysed cells. Asterisks indicate a statistical difference (***p < 0.001, Wilcoxon rank sum test).