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. 2015 Oct 5;5:14390. doi: 10.1038/srep14390

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(a) Effects of exogenous putrescine (Put) on ROS levels in the chloroplasts of cucumber plants grown in nutrient solutions with or without NaCl for 0, 3 and 7 days. The lower epidermis of the third leaves were torn off and incubated with 2′, 7′-dichlorofluorescein diacetate (DCF-DA). Changes of fluorescence intensity in stomatal cells were observed using a Leica TCS-SP confocal laser scanning microscope. (b) Intensity of DCF-DA fluorescence was analysed using Image Leica SP2 software and was expressed as relative value. Each histogram represents a mean ± SE of three independent experiments. Different letters indicate significant differences between treatments (P < 0.05).