Figure 5. Effect of exogenous putrescine (Put) on BN–SDS-PAGE of thylakoid membrane proteins in leaves of cucumber plants grown in nutrient solutions with or without 75 mM NaCl for 7 days.

This separation allowed the subunit patterns of the complexes to be visualised. Analysis of the BN-PAGE gel using Imagemaster 2D Platinum software revealed more than 60 CBB-stained protein spots associated with molecular masses of 14.4–116 kDa. Of these, 32 protein spots differentially regulated in response to NaCl and NaCl+Put treatment were excised from the gels and identified by MALDI-TOF-MS and LC-ESI-MS/MS. Additional information on these identified proteins is summarised in Table 2. (a) Cont, 0 mM NaCl + 0 mM Put; (b) Put, 0 mM NaCl+8 mM Put; (c) NaCl, 75 mM NaCl + 0 mM Put; (d) NaCl+Put, 75 mM NaCl + 8 mM Put.