Fig. 4.
As in FERONIA, MARIS is required to sustain root-hair growth. (A) Main roots with root hairs of 5-d-old mri-2, fer-4, and WT (Col-0 accession) seedlings (Left) and mri-1, fer-1, and WT (Ler accession) seedlings (Right) grown in agar. Arrows point to short, defective root hairs. (Scale bar: 1 mm.) (B) Quantification of root-hair lengths relative to A. Asterisks denote significant differences versus the appropriate control (P < 0.001, two-tailed unpaired Student's t test). (C, Left) WT, anx1 anx2, and mri-3D anx1 anx2 seedlings growing on the surface of a microagar plate. (Right) Pictures of primary roots and their root hairs. (Scale bars: 5 mm, Left; 1 mm, Right.) (D) Young root hairs in the root elongation zone of WT, mri-2, and mri-3D anx1 anx2 seedlings grown in liquid medium. Note the loss of CW integrity or CW overaccumulation (arrow) for mri-2 and mri-3D root hairs, respectively. (Scale bar: 20 μm.) See also Figs. S3 and S4 and Movie S1. (E) Main root of untransformed fer-4 seedling and fer-4 T1s expressing pMRI-MRIR240C-YFP. (Scale bar: 1 mm.)