Fig. 5.

Foxo1 deletion plus GSK3β inhibition rescues proliferation in CpG-treated BCR^neg B cells. Splenic B cells were sorted, labeled with CFSE, and cultured either in medium (green) or with the indicated stimuli, with (blue) or without (red) the GSK3β inhibitor BIO. BCR^pos, Foxo1f/+ and BCR^neg Foxo1Δ/+ cells were sorted from B1-8f/J_HT, CD21-Cre, Foxo1^f/+ mice, whereas BCR^neg, Foxo1Δ/Δ cells were sorted from B1-8f/J_HT, CD21-Cre, Foxo1^f/f mice. Data are representative of three biological replicates.