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Expression of Bcl-2 rescues survival of BCRneg B cells in vitro. Purified splenic B cells were transduced with TAT-Cre for 45 min and cultured in vitro. After 4 or 14 d, cells were analyzed for a change in the proportion of ToPro3−, CD19+ B cells that lost expression of IgM and IgD. Data are representative of three biological replicates.
