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Stimulation with CpG induces normal NF-κB DNA-binding activity in BCRneg B cells. BCRpos and BCRneg B cells from B1-8f/+, bcl-2tg mice were cultured overnight in 1% FCS-containing medium and stimulated for the indicated times with CpG. Nuclear lysates were used for EMSA. The data presented here were biologically replicated once, using the 0- and 120-min times.
