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. 2015 Sep 14;112(39):12199–12204. doi: 10.1073/pnas.1507145112

Fig. 4.

Fig. 4.

Lhx2 and β-Cat collaboratively induce Pax6 expression. (A) Quantitative RT-PCR to compare the relative expression level of Pax6 in E12.5 WT and cKO dTel. The expression of Pax6 is significantly down-regulated in the cKO (P < 0.05; n = 5). (B) The reporter constructs, Pax6p-Luc (containing Pax6 promoter) and EtelAPax6p-Luc (containing Pax6 promoter and a Pax6 enhancer, E, as indicated). Reporter activity without cotransfection of effectors was set as 1, and results are presented as fold of induction. The activity of Pax6p cannot be induced by Lhx2, but it can be induced by active βCat, and there is no further induction when Lhx2 and active βCat were both added (n.s, not significant). EtelAPax6p-Luc activity can be induced by Lhx2 and active βCat, individually. Lhx2 and active βCat together can further induce the activity of EtelAPax6p (P < 0.01; n = 3). (C) Model for Lhx2 and active βCat collaboratively regulate Pax6 expression.