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. 2015 Oct 5;5:14793. doi: 10.1038/srep14793

Figure 2. Mapping of UreB immunodominant epitopes.

Figure 2

(a) UreB-specific T cells expanded from immunized mice were screened for their specific IFN-γ response to 93 overlapping 18-mer UreB peptides at a final concentration of 5 μmol/l in an ICS assay. The locations of the identified 18-mer sequences are shown. The embedding data showed the responses of immunized mice and PBS control mice to the UreB peptide pool. (b) The 13-mer overlapping peptides within the UreB313–330 18-mers were screened (left). The 13-mer peptides within UreB403–420 and UreB409–426 were screened (middle). Peptides were titrated to compare their activities (right). (c) Th17 epitopes within the 93 overlapping 18-mer UreB peptides were identified using UreB-specific T cells expanded from immunized mice. The embedding data showed the responses of immunized mice and PBS control mice to the UreB peptide pool. (d) 13-mer peptides within UreB313–330 18-mers were screened as described in b (left). The 13-mer peptides within UreB403–420 and UreB409–426 were screened (middle). Peptides were titrated under serum-free conditions to compare their activities (right). The results are representative of five independent experiments. The data are expressed as the mean ± S.D. (n = 10). ***P < 0.001.